Susceptibility of suckling mice to variola virus.

Single-Dose Assay Technique for Variola

RILEY, JEAN M. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), AND MICHAEL D. ORLANDO. Singledose assay technique for variola virus. Appl. Microbiol. 12:7-9. 1964.-A biological assay for variola virus was needed that would (i) require a minimum of time and (ii) have acceptable precision. Preliminary titrations made in 11-day-old embryonated eggs and in suckling mice (6 to 24 ...

Single - Dose Assay Technique for Variola Virus " 2

RILEY, JEAN M. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), AND MICHAEL D. ORLANDO. Singledose assay technique for variola virus. Appl. Microbiol. 12:7-9. 1964.-A biological assay for variola virus was needed that would (i) require a minimum of time and (ii) have acceptable precision. Preliminary titrations made in 11-day-old embryonated eggs and in suckling mice (6 to 24 ...

Pathogenesis of vaccine strains of measles virus in suckling hamsters

Age dependence of viral expression: comparative pathogenesis of two rodent-adapted strains of measles virus in mice.

The pathogenesis of two rodent-adapted strains of measles virus was studied in 1- to 2-day-old suckling and 4-week-old weanling BALB/c mice. Both the mouse-adapted Edmonston (MAEd) strain and the hamster-neurotropic (HNT) strain caused necrotizing giant-cell encephalitis with a 90 to 100% mortality after intracerebral inoculation into suckling mice. After intracerebral inoculation into weanling...

Protection against lethal vaccinia virus challenge in HLA-A2 transgenic mice by immunization with a single CD8+ T-cell peptide epitope of vaccinia and variola viruses.

CD8(+) T lymphocytes have been shown to be involved in controlling poxvirus infection, but no protective cytotoxic T-lymphocyte (CTL) epitopes are defined for variola virus, the causative agent of smallpox, or for vaccinia virus. Of several peptides in vaccinia virus predicted to bind HLA-A2.1, three, VETFsm(498-506), A26L(6-14), and HRP2(74-82), were found to bind HLA-A2.1. Splenocytes from HL...